Serum Markers of Liver, Heart, Kidney, Lipid Profile and Histopathology in Female ODS Rats Treated with Nutrient Synergy
Roomi, M.W., Ivanov, V., Netke, S, Rath, M., and Niedzwieki, A
Matthias Rath, Inc., R&D, Cancer Division, Santa Clara, CA 95050
Presented at: American College of Nutrition, Nashville, Tennessee, October 9-12, 2003
Nutrient Synergy, a specific mixture of ascorbic acid, lysine, proline and green tea extract, has potent anti-metastatic activity. Studies were designed to investigate whether Nutrient Synergy (NS) has an adverse effect on liver, heart, and kidney functional serum enzymes, lipid profile, and histopathology. Adult female ODS rats weighing 250-300 gm were gavaged with NS 30, 90 and 150 mg, or with saline (control) daily for seven days. The rats were euthanized, blood was drawn, and the serum was analyzed for various biochemical parameters. Liver function: No difference was observed in protein, albumin (A), globulin (G), A/G ratio, alkaline phosphatase and aspartate aminotransferase (AST) between treated and control groups. However, alanine aminotransferase slowly decreased with increased dose of NS. Heart function: Lactate dehydrogenase, creatine kinase, and AST in test groups did not differ from control groups. Kidney function: Creatinine content was the same for the test and control groups. Uric acid however, showed a gradual non-significant increase, and BUN, a decrease, as did the B/C ratio, with an increased concentration of NS. Lipid profile: In test groups, both cholesterol and triglyceride levels decreased compared to control groups. Histopathology: Consistent with the serum markers, there was no difference in the histopathology of liver, kidney, heart, and lung compared to control groups and amongst test groups. These results demonstrate that Nutrient Synergy has neither adverse effects on vital organs nor on the associated serum enzymes, and is therefore, safe to use even at these high doses, which far exceed the normal dosage of the nutrient.
Nutrient Synergy has been shown in previous studies to have potent anti-metastatic activity. To address safety issues, our research team studied the effect of feeding different levels of this nutrient mixture for seven days on the clinical chemistry of adult female ODS rats, such as functional serum enzymes of the liver, heart, and kidney, as well as lipid profile and histopathology. Study results demonstrate that not only is Nutrient Synergy safe at higher doses, but also is associated with improved cellular functions in various organs in the body.