Cancer

Cellular Medicine in Cancer

Cellular Medicine provides a new perspective in the developmental steps of cancer and its metastasis and new safe, effective therapeutic options.

Choices and Outcomes in Cancer Treatment:

For decades, standard treatment for cancer has consisted of surgery, radiation and chemotherapy. Radiation and chemotherapy, the most frequently used therapies, not only are ineffective in providing a cure, but also indiscriminately attack all cells – healthy and cancerous, causing cellular damage and destruction of the body's connective tissue, the defense against cancer metastasis. Both radiation and chemotherapy trigger the development of new cancers and damage the immune system and body organs. In addition, these interventions activate enzymes that facilitate the release of cancer cells from a localized area to spread to other organs.

Cellular Health Research has achieved a breakthrough in cancer research by defining the cellular mechanisms involved in cancer proliferation and metastasis and developed a natural means of controlling these mechanisms. Efficient control of the spread of a disease by collagen-dissolving enzyme blocks has been successful with several diseases. This is especially important in diseases for which orthodox medicine has no preventive or healing therapies yet. A combination of natural nutrients formulated to support the body in curbing metastasis and reversing tumor growth, has been shown to be effective against a variety of human cancer cell lines, without adverse effects on normal cells. Please select a title below to read the respective research study .

To reproduce and spread to other parts in the body, cancer cells degrade the extracellular matrix (ECM) by secreting various matrix metalloproteinases (MMPs), which have been correlated with the aggressiveness of tumor growth. There is compelling evidence to suggest that MMP-2 and MMP-9 play important roles in tumor invasion and metastasis. This study demonstrated that the technique of co-culture assay offers a superior screening system for potential cancer drugs or agents that have either enhancing or inhibitory effects on MMPs.

How Cancer Spreads (Metastasis):

All forms of cancer spread with the help of a collagen dissolving mechanism. To reproduce and spread to other parts in the body, cancer cells degrade the extracellular matrix (ECM) by secreting various matrix metalloproteinases (MMPs), which have been correlated with the aggressiveness of tumor growth. With the help of these collagen-dissolving enzymes, cancer cells can bull doze their way though the extracellular matrix (ECM) and capsule enclosing the tumor and through an adjacent blood vessel wall, to be carried to other sites where the cancer cells can invade other organs, as shown below.

Cancer Metastasis – Normal cells become cancerous cells, which secrete matrix metalloproteinases (MMPS). MMPs destroy the extracellular matrix (ECM), enabling cancer cells to escape and spread to distal organs through the bloodstream.

Antitumorigenic Activity of Nutrient Synergy in Human Breast Cancer Lines MDA-MB-231 and MCF-7
Roomi, M.W., Ivanov, V., Rath, M. and Niedzwiecki, A.
Presented at: The 8th Annual Multidisciplinary Symposium on Breast Disease, Amelia Island, FL, February 13-16, 2003.
Breast cancer, worldwide the most prevalent cancer and the second leading cause of cancer deaths in women today (after lung cancer) metastasizes by the enzymatic destruction of surrounding connective tissue to metastasize. This study demonstrated that the nutrient mixture of lysine, proline, ascorbic acid and epigallocatechin gallate exerted potent synergistic antimetastatic effect on human breast MDA-MB-231 cancer cells by inhibiting MMP expression and Matrigel invasion. These results suggest that this nutrient formulation is a valuable and promising candidate for treating estrogen insensitive breast cancer.

Nutrient Synergy Counteracts Carcinogenic Activity of Estrogen in Cultured Human Cancer Cells Ivanov V., Roomi M.W., Niedzwiecki A., Rath M.
Presented at: American College of Nutrition, Nashville, Tennessee, October 9-12, 2003
Recent clinical studies have shown that hormone-replacement therapy (HRT) in menopausal women increases the risk of tumor development in estrogen-sensitive tissues. In this study, estradiol stimulated breast cancer (MCF-7) cell growth, MMP expression, matrix invasion, and VEGF secretion (measure of angiogenesis) in culture. These pro-carcinogenic effects of estradiol were significantly inhibited by Nutrient Synergy, suggesting NS is an excellent candidate for preventative and therapeutic use in the treatment of estrogen-related breast cancer.

Inhibition of Tumor Growth of Human Breast, Prostate, Colon and Melanoma Cancer Xenografts by Nutrient Synergy in Nude Mice
M.W. Roomi, N.W. Roomi, V. Ivanov, S.P. Netke, M. Rath and A. Niedzwiecki
Presented at: the 43rd Annual Meeting of The American Society for Cell Biology, San Francisco, CA, December 13-17, 2003
This study demonstrated the synergistic anticancer effect of lysine, proline, arginine, ascorbic acid and EGCG (from green tea extract) on human breast, colon, prostate, melanoma, fibrosarcoma, and synovial sarcoma cancer cell growth in nude mice without any adverse effects. Nude mice are are used since they are athymic, and thus are unable to mount most types of immune responses, including the ability to kill malignant cells by a cell-mediated immune response. The results of this study imply that Nutrient Synergy has great potential as a safe but effective therapeutic regimen for cancer treatment.

Nutrient Synergy – A Specific Formulation of Nutrients Containing Lysine, Proline, Ascorbic Acid, and Epigallocatechin Gallate Inhibits Matrix Metalloproteinases Activity and Invasion Potential of Human Cancer Cell Lines
M.W. Roomi, S.P. Netke, V. Ivanov, M. Rath and A. Niedzwiecki
Presented at: European Organization for Research and Treatment of Cancer (EORTC), AACR and NCI Symposium on Molecular Targets and Cancer Therapeutics, Frankfurt, Germany, Nov. 19-22, 2002
These results demonstrated that the synergistic effect of ascorbic acid, lysine, proline, and epigallocatechin gallate significantly inhibited metastasis potential of human melanoma, breast and liver cancer cells by inhibiting the expression of MMPs and Matrigel invasion, suggesting this non-toxic agent as a promising candidate for the treatment of human cancers.

Inhibitory Effects of Ascorbic Acid, Proline, and Lysine Supplementation on Matrigel Invasion by Human Breast Cancer Cells MDA-MB-231
S.P. Netke, V. Ivanov, M.W. Roomi, A. Niedzwiecki, M. Rath
Presented at: 19th Annual Miami Breast Cancer Conference, Miami Beach, Florida, February 27 – March 3, 2002.
The results from this study demonstrated that while an individual nutrient, such as ascorbic acid, can inhibit the invasion of cancer cells through Matrigel (a model for extracellular matrix), the synergistic effect of a combination of ascorbic acid, lysine, and proline significantly enhances inhibition of invasion. This implies that this nutrient mixture is a promising candidate for therapeutic use in the treatment of breast cancer cells, by blocking metastasis.

Melanoma

Metastatic and Cytotoxic Effects of Ascorbigen and iso-Ascorbigen in Human Cancer Cells
M.W. Roomi, A. Bogale, V. Ivanov, S. Netke, A. Niedzwiecki and M. Rath, M.D.
Presented at: American College of Nutrition, 43rd Annual Meeting, San Antonio, Texas, Oct. 3-6, 2002.
Diets high in cruciferous vegetables such as cabbage have been associated with prevention of certain types of cancers. In this study, ascorbigen, a major indole-containing compound in cabbage, was found to be toxic to human melanoma, liver, and colon cancer cell lines. In addition, it was found to inhibit cellular MMP expression, a measure of metastasis potential.

Nutrient Synergy – A Specific Formulation of Nutrients Containing Lysine, Proline, Ascorbic Acid and Epigallocatechin Gallate Inhibits Matrix Metalloproteinases Activity and Invasion Potential of Human Cancer Cell Lines
M.W. Roomi, S.P. Netke, V. Ivanov, M. Rath and A. Niedzwiecki
Presented at: European Organization for Research and Treatment of Cancer (EORTC), AACR and NCI Symposium on Molecular Targets and Cancer Therapeutics, Frankfurt, Germany, Nov. 19-22, 2002
These results demonstrated that the synergistic effect of ascorbic acid, lysine, proline, and epigallocatechin gallate significantly inhibited metastasis potential of human melanoma, breast and liver cancer cells by inhibiting the expression of MMPs and Matrigel invasion, suggesting this non-toxic agent as a promising candidate for the treatment of human cancers.

Cytotoxic Effect of Lipophilic Substitution at 2-, 6-, and 2,6-Positions in Ascorbic Acid and Expression of Matrix Metalloproteinases in Hep G2 Cells, Melanoma Cells and Normal Human Dermal Fibroblast
M.W. Roomi, S. Netke, V. Ivanov, A. Niedzwiecki and M. Rath
Presented at: American College of Nutrition, 42nd Annual Meeting, Orlando, Florida, Oct. 3-7, 2001.
Ascorbic acid and its derivatives have been shown to be cytotoxic and inhibit the growth of a number of malignant and non-malignant cell lines in culture and in animal models. In this study, ascorbic acid, which is water-soluble, was not toxic to the melanoma and liver cancer lines tested; however, the lipophilic (lipid soluble) derivatives studied were found to be markedly toxic to these cell lines. This implies that these lipid soluble derivatives, which can cross cell membranes and the blood brain barrier, have therapeutic potential in the treatment of cancer.Liver Cancer

Liver Cancer

Metastatic and Cytotoxic Effects of Ascorbigen and iso-Ascorbigen in Human Cancer Cells
M.W. Roomi, A. Bogale, V. Ivanov, S. Netke, A. Niedzwiecki and M. Rath, M.D.
Presented at: American College of Nutrition, 43rd Annual Meeting, San Antonio, Texas, Oct. 3-6, 2002
Diets high in cruciferous vegetables such as cabbage have been associated with prevention of certain types of cancers. In this study, ascorbigen, a major indole-containing compound in cabbage, was found to be toxic to human melanoma, liver, and colon cancer cell lines. In addition, it was found to inhibit cellular MMP expression, a measure of metastasis potential

Nutrient Synergy - A Specific Formulation of Nutrients Containing Lysine, Proline, Ascorbic Acid and Epigallocatechin Gallate Inhibits Matrix Metalloproteinases Activity and Invasion Potential of Human Cancer Cell Lines
M.W. Roomi, S.P. Netke, V. Ivanov, M. Rath and A. Niedzwiecki
Presented at: European Organization for Research and Treatment of Cancer (EORTC), AACR and NCI Symposium on Molecular Targets and Cancer Therapeutics, Frankfurt, Germany, Nov. 19-22, 2002
These results demonstrated that the synergistic effect of ascorbic acid, lysine, proline, and epigallocatechin gallate significantly inhibited metastasis potential of human melanoma, breast and liver cancer cells by inhibiting the expression of MMPs and Matrigel invasion, suggesting this non-toxic agent as a promising candidate for the treatment of human cancers.

Cytotoxic Effect of Lipophilic Substitution at 2-, 6-, and 2, 6-Positions in Ascorbic Acid and Expression of Matrix Metalloproteinases in Hep G2 Cells, Melanoma Cells and Normal Human Dermal Fibroblast
M.W. Roomi, S. Netke, V. Ivanov, A. Niedzwiecki and M. Rath
Presented at: American College of Nutrition, 42nd Annual Meeting, Orlando, Florida, Oct. 3-7, 2001
Ascorbic acid and its derivatives have been shown to be cytotoxic and inhibit the growth of a number of malignant and non-malignant cell lines in culture and in animal models. In this study, ascorbic acid, which is water-soluble, was not toxic to the melanoma and liver cancer lines tested; however, the lipophilic (lipid soluble) derivatives studied were found to be markedly toxic to these cell lines. This implies that these lipid soluble derivatives, which can cross cell membranes and the blood brain barrier, have therapeutic potential in the treatment of cancer.

Colon Cancer

Anti-Metastatic Activity of Nutrient Synergy on Human Colon Cancer Cell HCT 116
MW Roomi, V Ivanov, SP Netke, M Rath, and A Niedzwiecki
Presented at: International Research Conference on Food, Nutrition and Cancer, Washington D.C., July 17-18, 2003
Colon cancer, the second leading cause of cancer death in United States, results in 55, 000 deaths per year, mainly secondary to metastasis. In this study, NS was found to significantly inhibit the metastatic parameters of MMP-9 expression and Matrigel invasion without alteration in cell morphology. These results suggest that Nutrient Synergy not only has great antimetastatic potential as a therapeutic agent for colon cancer, but also is safe to use.

Metastatic and Cytotoxic Effects of Ascorbigen and iso-Ascorbigen in Human Cancer Cells
M.W. Roomi, A. Bogale, V. Ivanov, S. Netke, A. Niedzwiecki and M. Rath, M.D.
Presented at: American College of Nutrition, 43rd Annual Meeting, San Antonio, Texas, Oct. 3-6, 2002
Diets high in cruciferous vegetables such as cabbage have been associated with prevention of certain types of cancers. In this study, ascorbigen, a major indole-containing compound in cabbage, was found to be toxic to human melanoma, liver, and colon cancer cell lines. In addition, it was found to inhibit cellular MMP expression, a measure of metastasis potential.

Prostate Cancer

Pancreatic Cancer

Antitumor Effect of Nutrient Synergy: A Novel MMP Inhibitor in Pancreatic Cancer Cell Line MIA PaCa-2
M.W. Roomi, V. Ivanov, M.Rath and A. Niedzwiecki
Presented at: AACR-NCI-EORTC International Conference on Molecular Targets and Cancer Boston, MA, November 17-21, 2003.
Cancer of the pancreas is a highly lethal disease with the poorest likelihood of survival among all major malignancies; metastasis is associated with more than 80% of the cases. This study demonstrated significant inhibition of the metastatic parameters of MMP expression, invasion, and angiogenesis, suggesting NS is an excellent candidate for therapeutic use in the treatment of pancreatic cancer

Fibrosarcoma

Inhibitory Effect of Nutrient Synergy, a Specific Formulation of Nutrients Containing Lysine, Proline, Ascorbic Acid, and Epigallocatechin Gallate, on Matrix Metalloproteinase Activity and Invasion of Human Fibrosarcoma HT-1080 Cells
M.W. Roomi, V. Ivanov, S.P. Netke, M. Rath and A. Niedzwiecki
Presented at: FASEB (Federation of American Societies for Experimental Biology) Conference, San Diego, CA, April 11-15, 2003
To reproduce and spread to other parts in the body, cancer cells degrade the extracellular matrix (ECM) by secreting various matrix metalloproteinases (MMPs), which have been correlated with the aggressiveness of tumor growth. In this study, Nutrient Synergy, a specific mixture of nutrients, including lysine, proline, ascorbic acid, and epigallocatechin gallate, significantly inhibited the expression of both MMP-2 and MMP-9, and the invasion of human fibrosarcoma HT-1080 cells through Matrigel in a dose dependent fashion, without toxic effect to cells. These results suggest that Nutrient Synergy has great potential as a natural, non-toxic therapeutic regimen based on its antimetastatic activity.

Methodology

A Novel In Vitro Bioassay for Screening Matrix Metalloproteinase Activity in Human Cancer Cell Lines
M.W. Roomi, S.P. Netke, V. Ivanov, M. Rath and A. Niedzwiecki
Presented at: 94th Annual Meeting of AACR (American Association for Cancer Research), Washington D.C., July 11-14.
To reproduce and spread to other parts in the body, cancer cells degrade the extracellular matrix (ECM) by secreting various matrix metalloproteinases (MMPs), which have been correlated with the aggressiveness of tumor growth. There is compelling evidence to suggest that MMP-2 and MMP-9 play important roles in tumor invasion and metastasis. This study demonstrated that the technique of co-culture assay offers a superior screening system for potential cancer drugs or agents that have either enhancing or inhibitory effects on MMPs.

Sitemap